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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, L. J.; DUARTE, A. W. F.; ROSA, L. H.; OLIVEIRA, V. M.; MELO, I. S. de. |
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Afiliação: |
L. J. SILVA, ESALQ/USP; A. W. F. DUARTE, USP; L. H. ROSA, UFMG; V. M. OLIVEIRA, CPQBA/Unicamp. |
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Título: |
Fosmid library: methodological challenges. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest. MenosAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A p... Mostrar Tudo |
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Palavras-Chave: |
Antarctic peninsula; Metagenomic library; Unculturable microrganisms. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139515/1/2015RA-008.pdf
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Marc: |
LEADER 03101nam a2200193 a 4500
001 2037800
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, L. J.
245 $aFosmid library$bmethodological challenges.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1.$c0676
520 $aAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest.
653 $aAntarctic peninsula
653 $aMetagenomic library
653 $aUnculturable microrganisms
700 1 $aDUARTE, A. W. F.
700 1 $aROSA, L. H.
700 1 $aOLIVEIRA, V. M.
700 1 $aMELO, I. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
26/08/2002 |
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Data da última atualização: |
13/01/2016 |
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Autoria: |
XAVIER, A.; ANDRADE, H. B.; OLIVEIRA, M. L.; WENDLING, I. |
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Afiliação: |
Wendling, I., Pesquisador da Embrapa Florestas. |
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Título: |
Desempenho do enraizamento de microestacas e miniestacas de clones híbridos de Eucalyptus grandis. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Revista Árvore, Viçosa, v. 25, n. 4, p. 403-411, 2001. |
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Idioma: |
Português |
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Conteúdo: |
O presente trabalho objetivou avaliar o desempenho de micro e miniestacas de dois clones híbridos de Eucalyptus grandis quanto ao enraizamento, ao crescimento em altura e diâmetro do colo e à matéria seca da parte aérea e radicular aos 85 dias de idade da muda. Com base nas técnicas de mini e microestaquia, foram testados três períodos de permanência em casa de vegetação (20, 25 e 30 dias) e duas dosagens do regulador de crescimento para enraizamento AIB (O e 2.000 mg l-I) em mini e microestacas obtidas dos respectivos jardins clonais, Os resultados obtidos permitiram concluir que a técnica de microestaquia apresentou resultados superiores aos obtidos pela miniestaquia, principalmente em relação ao porcentual e à velocidade de enraizamento dos dois clones híbridos de Eucalyptus grandis avaliados. |
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Palavras-Chave: |
Clonal forestry; Microcuttings; Microestaquia; Minicuttings; Miniestaquia; Silvicultura clonal. |
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Thesagro: |
Eucalyptus Grandis; Propagação Vegetativa. |
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Thesaurus NAL: |
vegetative propagation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/38381/1/Desempenho-do-enraizamento-de-microestacas.pdf
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Marc: |
LEADER 01591naa a2200265 a 4500
001 1305205
005 2016-01-13
008 2001 bl uuuu u00u1 u #d
100 1 $aXAVIER, A.
245 $aDesempenho do enraizamento de microestacas e miniestacas de clones híbridos de Eucalyptus grandis.
260 $c2001
520 $aO presente trabalho objetivou avaliar o desempenho de micro e miniestacas de dois clones híbridos de Eucalyptus grandis quanto ao enraizamento, ao crescimento em altura e diâmetro do colo e à matéria seca da parte aérea e radicular aos 85 dias de idade da muda. Com base nas técnicas de mini e microestaquia, foram testados três períodos de permanência em casa de vegetação (20, 25 e 30 dias) e duas dosagens do regulador de crescimento para enraizamento AIB (O e 2.000 mg l-I) em mini e microestacas obtidas dos respectivos jardins clonais, Os resultados obtidos permitiram concluir que a técnica de microestaquia apresentou resultados superiores aos obtidos pela miniestaquia, principalmente em relação ao porcentual e à velocidade de enraizamento dos dois clones híbridos de Eucalyptus grandis avaliados.
650 $avegetative propagation
650 $aEucalyptus Grandis
650 $aPropagação Vegetativa
653 $aClonal forestry
653 $aMicrocuttings
653 $aMicroestaquia
653 $aMinicuttings
653 $aMiniestaquia
653 $aSilvicultura clonal
700 1 $aANDRADE, H. B.
700 1 $aOLIVEIRA, M. L.
700 1 $aWENDLING, I.
773 $tRevista Árvore, Viçosa$gv. 25, n. 4, p. 403-411, 2001.
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Embrapa Florestas (CNPF) |
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